Bafort, Q., Prost, L., Aydogdu Lohaus, E., Van de Vloet, A., Casteleyn, G., Van de Peer, Y., & De Clerck, O. (2023). Studying Whole-Genome Duplication Using Experimental Evolution of Chlamydomonas. In Y. Van de Peer (Ed.), Polyploidy (Vol. 2545, pp. 351–372). https://doi.org/10.1007/978-1-0716-2561-3_18
In this chapter, we present the use of Chlamydomonas reinhardtii in experiments designed to study the evolutionary impacts of whole genome duplication. We shortly introduce the algal species and depict why it is an excellent model for experimental evolution. Subsequently, we discuss the most relevant steps and methods in the design of a ploidy-related Chlamydomonas experiment. These steps include strain selection, ploidy determination, different methods of making diplo- and polyploid Chlamydomonas cells, replication, culturing conditions, preservation, and the ways to quantify phenotypic and genotypic change.